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Utilized
in medical science
and biology and in such diverse fields as materials development, metallic materials, ceramics, and semiconductors. SEM involves the manipulation
of an electron
beam that is scanned across the surface of specially prepared specimens to
obtain a greatly enlarged, high-resolution image of the specimen's exposed
structure. Specimens are scanned with a very fine probe ('tip') and the
strength of interaction between the tip and surface us monitored. The
specimen can be observed whole for assessing external structure or
freeze-fracture techniques can be used to image internal structures
Source
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